Direct imaging of uncoated biological samples enables correlation of super-resolution and electron microscopy data.

ABSTRACT: A simple method for imaging biological tissue samples by electron microscopy and its correlation with super-resolution light microscopy is presented. This room temperature protocol, based on protecting thin biological specimens with methylcellulose and imaging with low voltage scanning electron microscopy, circumvents complex classical electron microscopy sample preparation steps requiring dehydration, resin embedding and use of contrast agents. This technique facilitates visualization of subcellular structures e.g. synaptic clefts and synaptic vesicles in mouse brain tissue and the organization of mitochondrial cristae in the zebrafish retina. Application of immunogold protocols to these samples can determine the precise localization of synaptic proteins and, in combination with super-res

SUBMITTER: Mateos JM 

PROVIDER: S-EPMC6072772 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature