Specificities and Efficiencies of Primers Targeting Candidatus Phylum Saccharibacteria in Activated Sludge.

ABSTRACT: Candidatus Saccharibacteria is a well-described candidate phylum that has not been successfully isolated. Nevertheless, its presence was suggested by 16S rRNA gene sequencing data, and it is frequently detected in natural environments and activated sludge. Because pure culture representatives of Candidatus Saccharibacteria are lacking, the specificity of primers for the determination of their abundance and diversity should be carefully evaluated. In this study, eight Candidatus Saccharibacteria-specific primers were selected from previous studies and evaluated for their coverage against a public database, annealing temperature of the combined primer sets, as well as their utilization to determine the detection frequencies and phylogenetic diversity by cloning analysis,

SUBMITTER: Takenaka R 

PROVIDER: S-EPMC6073563 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature