Primary T Cells from Cutaneous T-cell Lymphoma Skin Explants Display an Exhausted Immune Checkpoint Profile.

ABSTRACT: Cutaneous T-cell lymphoma (CTCL) develops from clonally expanded CD4⁺ T cells in a background of chronic inflammation. Although dendritic cells (DCs) stimulate T cells and are present in skin, cutaneous T cells in CTCL do not respond with effective antitumor immunity. We evaluated primary T-cell and DC émigrés from epidermal and dermal explant cultures of skin biopsies from CTCL patients (n = 37) and healthy donors (n = 5). Compared with healthy skin, CD4⁺ CTCL populations contained more T cells expressing PD-1, CTLA-4, and LAG-3. CD8⁺ CTCL populations contained more T cells expressing CTLA-4 and LAG-3. CTCL populations also contained more T cells expressing the inducible T-cell costimulator (ICOS), a marker of T-cell activation. DC émigrés fr

SUBMITTER: Querfeld C 

PROVIDER: S-EPMC6074045 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature