Interrupted reprogramming into induced pluripotent stem cells does not rejuvenate human mesenchymal stromal cells.

ABSTRACT: Replicative senescence hampers application of mesenchymal stromal cells (MSCs) because it limits culture expansion, impairs differentiation potential, and hinders reliable standardization of cell products. MSCs can be rejuvenated by reprogramming into induced pluripotent stem cells (iPSCs), which is associated with complete erasure of age- and senescence-associated DNA methylation (DNAm) patterns. However, this process is also associated with erasure of cell-type and tissue-specific epigenetic characteristics that are not recapitulated upon re-differentiation towards MSCs. In this study, we therefore followed the hypothesis that overexpression of pluripotency factors under culture conditions that do not allow full reprogramming might reset senescence-associated changes without entering a p

SUBMITTER: Gobel C 

PROVIDER: S-EPMC6076311 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature