Genetic and genomic stability across lymphoblastoid cell line expansions.

ABSTRACT:

Objective

Lymphoblastoid cell lines are widely used in genetic and genomic studies. Previous work has characterized variant stability in transformed culture and across culture passages. Our objective was to extend this work to evaluate single nucleotide polymorphism and structural variation across cell line expansions, which are commonly used in biorepository distribution. Our study used DNA and cell lines sampled from six research participants. We assayed genome-wide genetic variants and inferred structural variants for DNA extracted from blood, from transformed cell cultures, and from three generations of expansions.

Results

Single nucleotide variation was stable between DNA and expanded cell lines (ranging from 99.90 to 99.98% concordance). Structural variation was less c

SUBMITTER: Scheinfeldt LB 

PROVIDER: S-EPMC6076395 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature