Studies of biochemical crosstalk in chromatin with semisynthetic histones.

ABSTRACT: Reversible post-translational modifications of histone proteins in eukaryotic chromatin are closely tied to gene function and cellular development. Specific combinations of histone modifications, or marks, are implicated in distinct DNA-templated processes mediated by a range of chromatin-associated enzymes that install, erase and interpret the histone code. Mechanistic studies of the precise biochemical relationship between sets of marks and their effects on chromatin function are significantly complicated by the dynamic nature and heterogeneity of marks in cellular chromatin. Protein semisynthesis is a chemical technique that enables the piecewise assembly of uniformly and site-specifically modified histones in quantities sufficient for biophysical and biochemical analyses. Recent pionee

SUBMITTER: Leonen CJA 

PROVIDER: S-EPMC6076846 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature