Development of a high throughput yeast-based screening assay for human carbonic anhydrase isozyme II inhibitors.

ABSTRACT: Carbonic anhydrase (CA; EC 4.2.1.1) catalyzes the reversible hydration of carbon dioxide (CO₂) to bicarbonate and proton. There are 16 known isozymes of α-CA in humans, which differ widely in their kinetics, subcellular localization and tissue-specific distribution. Several disorders are associated with abnormal levels of CA, and so the inhibition of CA has pharmacological application in the treatment of many diseases. Currently, searching for novel CA inhibitors (CAI) has been performed using in vitro methods, and so their toxicity remains unknown at the time of screening. To obtain potentially safer CAIs, a screening procedure using an in vivo assay seems to have more advantages. Here, we developed a yeast-based in vivo assay for the detection of inhibitors of the human CA iso

SUBMITTER: Sangkaew A 

PROVIDER: S-EPMC6076874 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature