High efficiency transformation of Brassica oleracea var. botrytis plants by Rhizobium rhizogenes.

ABSTRACT: Brassica oleracea var. botrytis, a very popular crop grown for its edible inflorescence, is bred only as a mutated annual cultivar and does not naturally occur in environment. Since cauliflower is still described as the most troublesome of all the B. oleracea vegetables regarding transformation processes, it is fully justified to focus on the improvement of tools for its genetic modifications. Here, we present a successful protocol for genetic transformation of cauliflower employing the process of agroinfection. The primary analysis of in vitro response of five cultivars allowed us to have chosen Pionier as the most promising cultivar; in consequence the Pionier was transformed via Rhizobium-mediated techniques in order to evaluate both, R. radiobacter (EHA 105, LBA 4404) and R. rhizogenes

SUBMITTER: Kowalczyk T 

PROVIDER: S-EPMC6077290 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature