Essential nucleotide- and protein-dependent functions of Actb/β-actin.

ABSTRACT: The highly similar cytoplasmic β- and γ-actins differ by only four functionally similar amino acids, yet previous in vitro and in vivo data suggest that they support unique functions due to striking phenotypic differences between Actb and Actg1 null mouse and cell models. To determine whether the four amino acid variances were responsible for the functional differences between cytoplasmic actins, we gene edited the endogenous mouse Actb locus to translate γ-actin protein. The resulting mice and primary embryonic fibroblasts completely lacked β-actin protein, but were viable and did not present with the most overt and severe cell and organismal phenotypes observed with gene knockout. Nonetheless, the edited mice exhibited progressive high-frequency hearing loss and dege

SUBMITTER: Patrinostro X 

PROVIDER: S-EPMC6077724 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature