Gambogic Acid and Its Analogs Inhibit Gap Junctional Intercellular Communication.

ABSTRACT: Gap junctions (GJs) are intercellular channels composed of connexins. Cellular molecules smaller than 1 kDa can diffuse through GJs by a process termed gap junctional intercellular communication (GJIC), which plays essential roles in various pathological and physiological conditions. Gambogic acid (GA), a major component of a natural yellow dye, has been used as traditional medicine and has been reported to have various therapeutic effects, including an anti-cancer effect. In this study, two different GJ assay methods showed that GA and its analogs inhibited GJIC. The inhibition was rapidly reversible and was not mediated by changes in surface expression or S368 phosphorylation of Cx43, cellular calcium concentration, or redox state. We also developed an assay system to measure the interce

SUBMITTER: Choi EJ 

PROVIDER: S-EPMC6077758 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature