Hypomethylation of GRHL3 gene is associated with the occurrence of neural tube defects.

ABSTRACT:

Aim

To investigate the relationship between GRHL3 methylation and the etiology of neural tube defects (NTDs).

Materials & methods

Analyze data from a genome-wide DNA methylation array. Targeted DNA methylation analysis was performed for 46 cases and 23 controls. At last, grhl3 overexpression and gene depletion experiments were conducted in zebrafish.

Results

Five hypomethylated CpGs were discovered in the methylation arrays performed on NTD cases. In a validation study, 15 hypomethylated CpGs were found and the overall methylation levels decreased in brain/spinal cord tissue from NTD cases. The knockdown and overexpression of grhl3 in zebrafish damaged embryonic convergent extension processes.

Conclusion

Hypomethylation of GRHL3 in central nervous tissue is ass

SUBMITTER: Tian T 

PROVIDER: S-EPMC6077761 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature