Melittin induces NSCLC apoptosis via inhibition of miR-183.

ABSTRACT:

Background

Non-small-cell lung cancer (NSCLC) has one of the highest mortality rates among cancers worldwide, with a poor prognosis. Previous studies have reported that melittin, an active component of apitoxin, exerts anti-inflammatory and antitumor effects via vascular endothelial growth factor or FoxO1.

Methods

CCK8, flow cytometry assay and Western blotting were performed to evaluate the effect of melittin on NSCLC.

Results

The present study demonstrates that melittin activated caspase-2 by inhibiting miR-183 expression and, thus, induced NSCLC apoptosis in both NCI-H441 cancer cell line assays and an in vivo xenograft model. The results of the cell-based assays showed that melittin (2 μg/mL) robustly suppressed miR-183 expression level and resulted in decreased i

SUBMITTER: Gao D 

PROVIDER: S-EPMC6078185 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature