Lower Fasted-State but Greater Increase in Muscle Protein Synthesis in Response to Elevated Plasma Amino Acids in Obesity.

ABSTRACT:

Objective

Obesity alters protein metabolism in skeletal muscle, but consistent evidence is lacking. This study compared muscle protein synthesis in adults with obesity and in lean controls in the fasted state and during an amino acid infusion.

Methods

Ten subjects with obesity (age: 36 ± 3 years; BMI: 34 ± 1 kg/m² ) and ten controls (age: 35 ± 3 years; BMI: 23 ± 1 kg/m² ) received an infusion of L-[2,3,3,4,5,5,5,6,6,6-² H₁₀ ]leucine (0.15 μmol/kg fat-free mass/min) to measure muscle protein synthesis after an overnight fast and during amino acid infusion.

Results

Despite greater muscle mammalian target of rapamycin phosphorylation (P ≤ 0.05), fasted-state mixed-muscle and mitochondrial protein synthesis were lower in subject

SUBMITTER: Tran L 

PROVIDER: S-EPMC6078204 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature