Using low-coverage whole genome sequencing technique to analyze the chromosomal copy number alterations in the exfoliative cells of cervical cancer.

ABSTRACT:

Objectives

We analyzed the chromosomal-arm-level copy number alterations (CNAs) in the cervical exfoliative cell and tissue samples by using the low-coverage whole genomic sequencing technique.

Methods

In this study, we retrospectively collected 55 archived exfoliated cervical cell suspension samples and the corresponding formalin-fixed and paraffin-embedded tissue section samples including 27 invasive cervical cancer and 28 control cases. We also collected 19 samples of the cervical exfoliative cells randomly from women to verify the new algorithm model. We analyzed the CNAs in cervical exfoliated cell and tissue samples by using the low-coverage next generation of sequencing.

Results

In the model-building study, multiple chromosomal-arm-level CNAs were detected in b

SUBMITTER: Ren T 

PROVIDER: S-EPMC6078888 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature