Hybrid cell reactor system from Escherichia coli protoplast cells and arrayed lipid bilayer chamber device.

ABSTRACT: We developed a novel hybrid cell reactor system via functional fusion of single Escherichia coli protoplast cells, that are deficient in cell wall and expose plasma membrane, with arrayed lipid bilayer chambers on a device in order to incorporate the full set of cytosolic and membrane constituents into the artificial chambers. We investigated gene expression activity to represent the viability of the hybrid cell reactors: over 20% of hybrid cells showed gene expression activity from plasmid or mRNA. This suggests that the hybrid cell reactors retained fundamental activity of genetic information transduction. To expand the applicability of the hybrid cell reactors, we also developed the E. coli-in-E. coli cytoplasm system as an artificial parasitism system. Over 30% of encapsulated E. coli

SUBMITTER: Moriizumi Y 

PROVIDER: S-EPMC6078950 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature