Apurinic/apyrimidinic (AP) endonuclease 1 processing of AP sites with 5' mismatches.

ABSTRACT: Despite the DNA duplex being central to biological functions, many intricacies of this molecule, including the dynamic nature of mismatched base pairing, are still unknown. The unique conformations adopted by DNA mismatches can provide insight into the forces at play between nucleotides. Moreover, DNA-binding proteins apply their own individualized steric and electrochemical influences on the nucleotides that they interact with, further altering base-pairing conformations. Here, seven X-ray crystallographic structures of the human nuclease apurinic/apyrimidinic (AP) endonuclease 1 (APE1) in complex with its substrate target flanked by a 5' mismatch are reported. The structures reveal how APE1 influences the conformations of a variety of different mismatched base pairs. Purine-purine mismat

SUBMITTER: Fairlamb MS 

PROVIDER: S-EPMC6079627 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature