Functional analysis of Brassica napus phloem protein and ribonucleoprotein complexes.

ABSTRACT: Phloem sap contains a large number of macromolecules, including proteins and RNAs from different classes. Proteome analyses of phloem samples from different plant species under denaturing conditions identified hundreds of proteins potentially involved in diverse processes. Surprisingly, these studies also found a significant number of ribosomal and proteasomal proteins. This led to the suggestion that active ribosome and proteasome complexes might be present in the phloem, challenging the paradigm that protein synthesis and turnover are absent from the enucleate sieve elements of angiosperms. However, the existence of such complexes has as yet not been demonstrated. In this study we used three-dimensional gel electrophoresis to separate several protein complexes from native phloem sap from

SUBMITTER: Ostendorp A 

PROVIDER: S-EPMC6079638 | biostudies-literature | 2017 May

REPOSITORIES: biostudies-literature