Dataset Information

Comparative root transcriptome of wild Arachis reveals NBS-LRR genes related to nematode resistance.

ABSTRACT:

Background

The Root-Knot Nematode (RKN), Meloidogyne arenaria, significantly reduces peanut grain quality and yield worldwide. Whilst the cultivated species has low levels of resistance to RKN and other pests and diseases, peanut wild relatives (Arachis spp.) show rich genetic diversity and harbor high levels of resistance to many pathogens and environmental constraints. Comparative transcriptome analysis can be applied to identify candidate resistance genes.

Results

Transcriptome analysis during the early stages of RKN infection of two peanut wild relatives, the highly RKN resistant Arachis stenosperma and the moderately susceptible A. duranensis, revealed genes related to plant immunity with contrasting expression profiles. These included genes involved in hormone signaling and secondary metabolites production and also members of the NBS-LRR class of plant disease resistance (R) genes. From 345 NBS-LRRs identified in A.duranensis reference genome, 52 were differentially expressed between inoculated and control samples, with the majority occurring in physical clusters unevenly distributed on eight chromosomes with preferential tandem duplication. The majority of these NBS-LRR genes showed contrasting expression behaviour between A. duranensis and A. stenosperma, particularly at 6 days after nematode inoculation, coinciding with the onset of the Hypersensitive Response in the resistant species. The physical clustering of some of these NBS-LRR genes correlated with their expression patterns in the contrasting genotypes. Four NBS-LRR genes exclusively expressed in A. stenosperma are located within clusters on chromosome Aradu. A09, which harbors a QTL for RKN resistance, suggesting a functional role for their physical arrangement and their potential involvement in this defense response.

Conclusion

The identification of functional novel R genes in wild Arachis species responsible for triggering effective defense cascades can contribute to the crop genetic improvement and enhance peanut resilience to RKN.

SUBMITTER: Mota APZ

PROVIDER: S-EPMC6080386 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature

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Publications

Comparative root transcriptome of wild Arachis reveals NBS-LRR genes related to nematode resistance.

Mota Ana Paula Zotta APZ Vidigal Bruna B Danchin Etienne G J EGJ Togawa Roberto Coiti RC Leal-Bertioli Soraya C M SCM Bertioli David John DJ Araujo Ana Claudia Guerra ACG Brasileiro Ana Cristina Miranda ACM Guimaraes Patricia Messenberg PM

BMC plant biology 20180806 1

<h4>Background</h4>The Root-Knot Nematode (RKN), Meloidogyne arenaria, significantly reduces peanut grain quality and yield worldwide. Whilst the cultivated species has low levels of resistance to RKN and other pests and diseases, peanut wild relatives (Arachis spp.) show rich genetic diversity and harbor high levels of resistance to many pathogens and environmental constraints. Comparative transcriptome analysis can be applied to identify candidate resistance genes.<h4>Results</h4>Transcriptome ...[more]

PMID: 30081841

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Project description:IntroductionNBS-LRR genes (NLRs) are the most extensive category of plant resistance genes (R genes) and play a crucial role in pathogen defense. Understanding the diversity and evolutionary dynamics of NLRs in different plant species is essential for improving disease resistance. This study investigates the NLR gene family in eight diploid wild strawberry species to explore their structural characteristics, evolutionary relationships, and potential for enhancing disease resistance.MethodsWe conducted a comprehensive genome-wide identification and structural analysis of NLRs across eight diploid wild strawberry species. Phylogenetic analysis was performed to examine the relationships between TIR-NLRs (TNLs), Non-TIR-NLRs (non-TNLs), CC-NLRs (CNLs), and RPW8-NLRs (RNLs). Gene structures were compared, and gene expression was profiled across different NLR subfamilies. Additionally, in vitro leaf inoculation assays with Botrytis cinerea were performed to assess the resistance of various strawberry species.ResultsOur analysis revealed that non-TNLs constitute over 50% of the NLR gene family in all eight strawberry species, surpassing the proportion of TNLs. Phylogenetic analysis showed that TNLs diverged into two subclades: one grouping with CNLs and the other closely related to RNLs. A significantly higher number of non-TNLs were under positive selection compared to TNLs, indicating their rapid diversification. Gene structure analysis demonstrated that non-TNLs have shorter gene structures than TNLs and exhibit higher expression levels, particularly RNLs. Notably, non-TNLs showed dominant expression under both normal and infected conditions. In vitro leaf inoculation assays revealed that Fragaria pentaphylla and Fragaria nilgerrensis, which have the highest proportion of non-TNLs, exhibited significantly greater resistance to Botrytis cinerea compared to Fragaria vesca, which has the lowest proportion of non-TNLs.DiscussionThe findings of this study provide important insights into the evolutionary dynamics of NLRs in strawberries, particularly the significant role of non-TNLs in pathogen defense. The rapid diversification and higher expression levels of non-TNLs suggest their potential contribution to enhanced disease resistance. This research highlights the value of non-TNLs in strawberry breeding programs aimed at improving resistance to pathogens such as Botrytis cinerea.

Dataset Information

Comparative root transcriptome of wild Arachis reveals NBS-LRR genes related to nematode resistance.

Background

Results

Conclusion

Publications

Comparative root transcriptome of wild Arachis reveals NBS-LRR genes related to nematode resistance.

Similar Datasets

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