Evidence that Lokern virus (family Peribunyaviridae) is a reassortant that acquired its small and large genome segments from Main Drain virus and its medium genome segment from an undiscovered virus.

ABSTRACT:

Background

Lokern virus (LOKV) is a poorly characterized arthropod-borne virus belonging to the genus Orthobunyavirus (family Peribunyaviridae). All viruses in this genus have tripartite, single-stranded, negative-sense RNA genomes, and the three RNA segments are designated as small, (S), medium (M) and large (L). A 559 nt. region of the M RNA segment of LOKV has been sequenced and there are no sequence data available for its S or L RNA segments. The purpose of this study was to sequence the genome of LOKV.

Methods

The genome of LOKV was fully sequenced by unbiased high-throughput sequencing, 5' and 3' rapid amplification of cDNA ends, reverse transcription-polymerase chain reaction and Sanger sequencing.

Results

The S and L RNA segments of LOKV consist of 952 and 686

SUBMITTER: Tangudu CS 

PROVIDER: S-EPMC6080421 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature