Live imaging of collagen deposition during skin development and repair in a collagen I - GFP fusion transgenic zebrafish line.

ABSTRACT: Fibrillar collagen is a major component of many tissues but has been difficult to image in vivo using transgenic approaches because of problems associated with establishing cells and organisms that generate GFP-fusion collagens that can polymerise into functional fibrils. Here we have developed and characterised GFP and mCherry collagen-I fusion zebrafish lines with basal epidermal-specific expression. We use these lines to reveal the dynamic nature of collagen-I fibril deposition beneath the developing embryonic epidermis, as well as the repair of this collagen meshwork following wounding. Transmission electron microscope studies show that these transgenic lines faithfully reproduce the collagen ultrastructure present in wild type larval skin. During skin development we show that collagen

SUBMITTER: Morris JL 

PROVIDER: S-EPMC6080847 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature