Tracking the sarcoplasmic reticulum membrane voltage in muscle with a FRET biosensor.

ABSTRACT: Ion channel activity in the plasma membrane of living cells generates voltage changes that are critical for numerous biological functions. The membrane of the endoplasmic/sarcoplasmic reticulum (ER/SR) is also endowed with ion channels, but whether changes in its voltage occur during cellular activity has remained ambiguous. This issue is critical for cell functions that depend on a Ca2+ flux across the reticulum membrane. This is the case for contraction of striated muscle, which is triggered by opening of ryanodine receptor Ca2+ release channels in the SR membrane in response to depolarization of the transverse invaginations of the plasma membrane (the t-tubules). Here, we use targeted expression of voltage-sensitive fluorescence resonance energy transfer (FRET) probes of the Mermaid fam

SUBMITTER: Sanchez C 

PROVIDER: S-EPMC6080890 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature