Ca²⁺-mitochondria axis drives cell division in hematopoietic stem cells.

ABSTRACT: Most of the hematopoietic stem cells (HSCs) within the bone marrow (BM) show quiescent state with a low mitochondrial membrane potential (ΔΨ_m). In contrast, upon stress hematopoiesis, HSCs actively start to divide. However, the underlying mechanism for the initiation of HSC division still remains unclear. To elucidate the mechanism underlying the transition of cell cycle state in HSCs, we analyzed the change of mitochondria in HSCs after BM suppression induced by 5-fluoruracil (5-FU). We found that HSCs initiate cell division after exhibiting enhanced ΔΨ_m as a result of increased intracellular Ca²⁺ level. Although further activation of Ca²⁺-mitochondria pathway led to loss of HSCs after cell division, the appropriate suppression of intracellular

SUBMITTER: Umemoto T 

PROVIDER: S-EPMC6080917 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature