Mammary molecular portraits reveal lineage-specific features and progenitor cell vulnerabilities.

ABSTRACT: The mammary epithelium depends on specific lineages and their stem and progenitor function to accommodate hormone-triggered physiological demands in the adult female. Perturbations of these lineages underpin breast cancer risk, yet our understanding of normal mammary cell composition is incomplete. Here, we build a multimodal resource for the adult gland through comprehensive profiling of primary cell epigenomes, transcriptomes, and proteomes. We define systems-level relationships between chromatin-DNA-RNA-protein states, identify lineage-specific DNA methylation of transcription factor binding sites, and pinpoint proteins underlying progesterone responsiveness. Comparative proteomics of estrogen and progesterone receptor-positive and -negative cell populations, extensive target validation

SUBMITTER: Casey AE 

PROVIDER: S-EPMC6080920 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature