Purinyl-cobamide is a native prosthetic group of reductive dehalogenases.

ABSTRACT: Cobamides such as vitamin B₁₂ are structurally conserved, cobalt-containing tetrapyrrole biomolecules that have essential biochemical functions in all domains of life. In organohalide respiration, a vital biological process for the global cycling of natural and anthropogenic organohalogens, cobamides are the requisite prosthetic groups for carbon-halogen bond-cleaving reductive dehalogenases. This study reports the biosynthesis of a new cobamide with unsubstituted purine as the lower base and assigns unsubstituted purine a biological function by demonstrating that Coα-purinyl-cobamide (purinyl-Cba) is the native prosthetic group in catalytically active tetrachloroethene reductive dehalogenases of Desulfitobacterium hafniense. Cobamides featuring different lower bases are not fun

SUBMITTER: Yan J 

PROVIDER: S-EPMC6081238 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature