Quantitative and multiplex microRNA assays from unprocessed cells in isolated nanoliter well arrays.

ABSTRACT: MicroRNAs (miRNAs) have recently emerged as promising biomarkers for the profiling of diseases. Translation of miRNA biomarkers to clinical practice, however, remains a challenge due to the lack of analysis platforms for sensitive, quantitative, and multiplex miRNA assays that have simple and robust workflows suitable for translation. The platform we present here utilizes functionalized hydrogel posts contained within isolated nanoliter well reactors for quantitative and multiplex assays directly from unprocessed cell samples without the need of prior nucleic acid extraction. Simultaneous reactor isolation and delivery of miRNA extraction reagents is achieved by sealing an array of wells containing the functionalized hydrogel posts and cells against another array of wells containing lysis

SUBMITTER: Tentori AM 

PROVIDER: S-EPMC6081239 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature