Protease activated receptor 2 (PAR2) activation causes migraine-like pain behaviors in mice.

ABSTRACT:

Background

Pain is the most debilitating symptom of migraine. The cause of migraine pain likely requires activation of meningeal nociceptors. Mast cell degranulation, with subsequent meningeal nociceptor activation, has been implicated in migraine pathophysiology. Degranulating mast cells release serine proteases that can cleave and activate protease activated receptors. The purpose of these studies was to investigate whether protease activated receptor 2 is a potential generator of nociceptive input from the meninges by using selective pharmacological agents and knockout mice.

Methods

Ratiometric Ca⁺⁺ imaging was performed on primary trigeminal and dural cell cultures after application of 2at-LIGRL-NH₂, a specific protease activated receptor 2 agonist.

SUBMITTER: Hassler SN 

PROVIDER: S-EPMC6081257 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature