Oligomerization of bacterially expressed H1N1 recombinant hemagglutinin contributes to protection against viral challenge.

ABSTRACT: Vaccination is the most effective intervention to prevent influenza and control the spread of the virus. Alternatives are needed to the traditional egg-based vaccine strategy for a more rapid response to new outbreaks. Two different hemagglutinin (HA) fragments (rHA1_1-326 and rHA1_53-269) derived from influenza A virus subtype H1N1 were expressed in Escherichia coli and characterized by immunoblot, gel filtration, hemagglutination, and competitive binding assays. rHA1_1-326 included neutralizing epitopes and the trimerization domain, whereas rHA1_53-269 included only the head of HA with the neutralizing epitopes. Mice were immunized with rHA1_1-326 or rHA1_53-269, and sera were tested for the presence of neutralizing antibodies.

SUBMITTER: Kuenstling TE 

PROVIDER: S-EPMC6081378 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature