Superoxide Anion Production and Bioenergetic Profile in Young and Elderly Human Primary Myoblasts.

ABSTRACT: Sarcopenia is the age-related loss of skeletal muscle mass, strength, and function. It is associated with regenerative difficulties by satellite cells, adult muscle stem cells, and alteration of oxidative management, mainly the increase in superoxide anions (O₂^•-). We aimed to investigate the relation between regenerative deficit in elderly and increase in O₂^•- production along with mitochondrial alterations. Myoblasts and myotubes from skeletal muscle of young and elderly healthy subjects (27.8 ± 6 and 72.4 ± 6.5 years old) were measured: (1) superoxide dismutase activity and protein content, (2) mitochondrial O₂^•- production levels, (3) O₂^•- production variability, and (4) mitochondrial bioenergetic p

SUBMITTER: Marrone M 

PROVIDER: S-EPMC6081572 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature