Leishmania donovani induced Unfolded Protein Response delays host cell apoptosis in PERK dependent manner.

ABSTRACT:

Background

Endoplasmic reticulum (ER) stress generated unfolded stress response (UPR) is a basic survival mechanism which protects cell under unfavourable conditions. Leishmania parasite modulates host macrophages in various ways to ensure its survival. Modulation of PI3K-Akt pathway in delayed apoptotic induction of host; enables parasite to stabilize the infection for further propagation.

Methodology

Infected RAW macrophages were exposed to campothecin or thagsigargin and phosphorylation status of PERK, Akt, BAD and Cyt-C was determined through western blotting using phospho specific antibody. Expression at transcriptional level for cIAP1 &2, ATF4, CHOP, ATF3, HO-1 and sXBP1 was determined using real time PCR. For inhibition studies, RAW macrophages were pre-treated with P

SUBMITTER: Abhishek K 

PROVIDER: S-EPMC6081962 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature