Mettl14 Is Essential for Epitranscriptomic Regulation of Striatal Function and Learning.

ABSTRACT: N⁶-methyladenosine (m⁶A) regulates mRNA metabolism and translation, serving as an important source of post-transcriptional regulation. To date, the functional consequences of m⁶A deficiency within the adult brain have not been determined. To achieve m⁶A deficiency, we deleted Mettl14, an essential component of the m⁶A methyltransferase complex, in two related yet discrete mouse neuronal populations: striatonigral and striatopallidal. Mettl14 deletion reduced striatal m⁶A levels without altering cell numbers or morphology. Transcriptome-wide profiling of m⁶A-modified mRNAs in Mettl14-deleted striatum revealed downregulation of similar striatal mRNAs encoding neuron- and synapse-specific proteins in both neuronal ty

SUBMITTER: Koranda JL 

PROVIDER: S-EPMC6082022 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature