Microfluidic diafiltration-on-chip using an integrated magnetic peristaltic micropump.

ABSTRACT: Diafiltration is a membrane filtration technique that rapidly removes permeable molecules from a solution by controlling the tangential and orthogonal flows over a membrane and by replenishing the permeate with an equivalent amount of replacement buffer. However, its application to the purification of many key biomaterials and nanomaterials has been limited by the large dead volume (>10 mL) that is required to automate the process. To address this challenge, we have developed a diafiltration-on-a-chip device that can process low-volume samples (50 μL). The key innovation of this device is a magnetically-driven on-chip peristaltic pump that is able to continuously drive fluid flow at rates as high as 50 mL h^-1 with minimal external instrumentation and a dead volume of <50 μL. To

SUBMITTER: Liu JF 

PROVIDER: S-EPMC6082132 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature