Comparative optical genome analysis of two pangolin species: Manis pentadactyla and Manis javanica.

ABSTRACT:

Background

The pangolin is a Pholidota mammal with large keratin scales protecting its skin. Two pangolin species ( Manis pentadactyla and Manis javanica ) have been recorded as critically endangered on the International Union for Conservation of Nature Red List of Threatened Species. Optical mapping constructs high-resolution restriction maps from single DNA molecules for genome analysis at the megabase scale and to assist genome assembly. Here, we constructed restriction maps of M. pentadactyla and M. javanica using optical mapping to assist with genome assembly and analysis of these species.

Findings

Genomic DNA was nicked with Nt.BspQI and then labeled using fluorescently labeled bases that were detected by the Irys optical mapping system. In total, 3,313,734 DNA molecul

SUBMITTER: Zhihai H 

PROVIDER: S-EPMC6082621 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature