Purification and characterization of monoclonal IgG antibodies recognizing Ebola virus glycoprotein.

ABSTRACT:

Purpose

The goal of this study was to purify and characterize Ebola virus glycoprotein (GP)-specific IgG antibodies from hybridoma clones.

Materials and methods

For hybridoma production, mice were injected by intramuscular-electroporation with GP DNA vaccines, and boosted with GP vaccines. The spleen cells were used for producing GP-specific hybridoma. Enzyme-linked immunosorbent assay, Western blot assay, flow cytometry, and virus-neutralizing assay were used to test the ability of monoclonal IgG antibodies to recognize GP and neutralize Ebola virus.

Results

Twelve hybridomas, the cell supernatants of which displayed GP-binding activity by enzyme-linked immunosorbent assay and the presence of both IgG heavy and light chains by Western blot assay, were chosen as a pos

SUBMITTER: Han BS 

PROVIDER: S-EPMC6082671 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature