IgE Cross-Reactivity between Humulus japonicus and Humulus lupulus.

ABSTRACT:

Purpose

Japanese hop (Humulus japonicus) is a major cause of weed pollinosis in East Asia. However, supplies of commercial allergen extract from this plant have not met clinical demand. The pollen of common hop (Humulus lupulus), a closely related species, may provide an alternative source if there is strong IgE cross-reactivity between these two species. We aimed to compare the IgE cross-reactivity and allergenicity of common hop and Japanese hop pollen.

Materials and methods

Cross-reactivity was measured by inhibition ELISA. One- and two-dimensional (2D) gel analyses combined with IgE immunoblotting and mass spectrometry [liquid chromatography coupled to electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS)] were performed to detect IgE-reactive pollen components

SUBMITTER: Jeong KY 

PROVIDER: S-EPMC6082984 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature