Bacterial Cytological Profiling Reveals the Mechanism of Action of Anticancer Metal Complexes.

ABSTRACT: Target identification and mechanistic studies of cytotoxic agents are challenging processes that are both time-consuming and costly. Here we describe an approach to mechanism of action studies for potential anticancer compounds by utilizing the simple prokaryotic system, E. coli, and we demonstrate its utility with the characterization of a ruthenium polypyridyl complex [Ru(bpy)₂dmbpy²⁺]. Expression of the photoconvertible fluorescent protein Dendra2 facilitated both high throughput studies and single-cell imaging. This allowed for simultaneous ratiometric analysis of inhibition of protein production and phenotypic investigations. The profile of protein production, filament size and population, and nucleoid morphology revealed important differences between inorganic a

SUBMITTER: Sun Y 

PROVIDER: S-EPMC6083414 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature