F-CphI represents a new homing endonuclease family using the Endo VII catalytic motif.

ABSTRACT:

Background

There are six known families of homing endonucleases, LAGLIDADG, GIY-YIG, HNH, His-Cys box, PD-(D/E)-XK, and EDxHD, which are characterized by their conserved residues. Previously, we discovered a novel homing endonuclease F-CphI encoded by ORF177 of cyanophage S-PM2. F-CphI does not resemble any characterized homing endonucleases. Instead, the C-terminus of F-CphI aligns well with the N-terminal catalytic domain of a Holliday junction DNA resolvase, phage T4 endonuclease VII (Endo VII).

Results

A PSI-BLAST search resulted in a total of 313 Endo VII motif-containing sequences in sequenced genomes. Multiple sequence alignment showed that the catalytically important residues of T4 Endo VII were all well conserved in these proteins. Our site-directed mutagenesis stud

SUBMITTER: Fang X 

PROVIDER: S-EPMC6083498 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature