PIPEBAR and OverlapPER: tools for a fast and accurate DNA barcoding analysis and paired-end assembly.

ABSTRACT:

Background

Taxonomic identification of plants and insects is a hard process that demands expert taxonomists and time, and it's often difficult to distinguish on morphology only. DNA barcodes allow a rapid species discovery and identification and have been widely used for taxonomic identification by targeting known gene regions that permit to discriminate these species. DNA barcode sequence analysis is usually carried out with processes and tools that still demand a high interaction with the user or researcher. To reduce at most such interaction, we proposed PIPEBAR, a pipeline for DNA chromatograms analysis of Sanger platform sequencing, ensuring high quality consensus sequences along with efficient running time. We also proposed a paired-end reads assembly tool, OverlapPER, which

SUBMITTER: Oliveira RRM 

PROVIDER: S-EPMC6083499 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature