Fate of the UPR marker protein Kar2/Bip and autophagic processes in fed-batch cultures of secretory insulin precursor producing Pichia pastoris.

ABSTRACT:

Background

Secretory recombinant protein production with Pichia (syn. Komagataella) pastoris is commonly associated with the induction of an unfolded protein response (UPR) usually apparent through increased intracellular levels of endoplasmic reticulum (ER) resident chaperones such as Kar2/Bip. During methanol-induced secretory production of an insulin precursor (IP) under industrially relevant fed-batch conditions the initially high level of intracellular Kar2/Bip after batch growth on glycerol unexpectedly declined in the following methanol fed-batch phase misleadingly suggesting that IP production had a low impact on UPR activation.

Results

Analysis of the protein production independent level of Kar2/Bip revealed that high Kar2/Bip levels were reached in the exponential

SUBMITTER: Roth G 

PROVIDER: S-EPMC6083527 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature