CNS-border associated macrophages respond to acute ischemic stroke attracting granulocytes and promoting vascular leakage.

ABSTRACT: The central nervous system (CNS) contains several types of immune cells located in specific anatomic compartments. Macrophages reside at the CNS borders surrounding the brain vessels, in leptomeningeal spaces and the choroid plexus, where they interact with the vasculature and play immunological surveillance and scavenging functions. We investigated the phenotypic changes and role of these macrophages in response to acute ischemic stroke. Given that CD163 expression is a hallmark of perivascular and meningeal macrophages in the rat and human brain, we isolated CD163⁺ brain macrophages by fluorescence activated cell sorting. We obtained CD163⁺ cells from control rats and 16 h following transient middle cerebral artery occlusion, after verifying that infiltration of CD1

SUBMITTER: Pedragosa J 

PROVIDER: S-EPMC6083589 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature