Optimizing the composition of a synthetic cellulosome complex for the hydrolysis of softwood pulp: identification of the enzymatic core functions and biochemical complex characterization.

ABSTRACT:

Background

The development of efficient cellulase blends is a key factor for cost-effectively valorizing biomass in a new bio-economy. Today, the enzymatic hydrolysis of plant-derived polysaccharides is mainly accomplished with fungal cellulases, whereas potentially equally effective cellulose-degrading systems from bacteria have not been developed. Particularly, a thermostable multi-enzyme cellulase complex, the cellulosome from the anaerobic cellulolytic bacterium Clostridium thermocellum is promising of being applied as cellulolytic nano-machinery for the production of fermentable sugars from cellulosic biomass.

Results

In this study, 60 cellulosomal components were recombinantly produced in E. coli and systematically permuted in synthetic complexes to study

SUBMITTER: Leis B 

PROVIDER: S-EPMC6083626 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature