A selective and sensitive method for quantification of endogenous polysulfide production in biological samples.

ABSTRACT: Hydrogen sulfide (H₂S) is a gasotransmitter that regulates cellular homeostasis and impacts on multiple physiological and pathophysiological processes. However, it exerts many of its biological actions indirectly via the formation of H₂S-derived sulfane sulfur species/polysulfides. Because of the high reactivity of sulfur species, the detection of H₂S-derived polysulfides in biological systems is challenging and currently used methods are neither sensitive nor quantitative. Herein, we describe a LC-MS/MS-based method that makes use of Sulfane Sulfur Probe 4 to detect endogenously generated polysulfides in biological samples in a selective, sensitive and quantitative manner. The results indicate a large variability in the activity of the H₂S-gener

SUBMITTER: Bibli SI 

PROVIDER: S-EPMC6083819 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature