Nimbus: a design-driven analyses suite for amplicon-based NGS data.

ABSTRACT:

Motivation

PCR-based DNA enrichment followed by massively parallel sequencing is a straightforward and cost effective method to sequence genes up to high depth. The full potential of amplicon-based sequencing assays is currently not achieved as analysis methods do not take into account the source amplicons of the detected variants. Tracking the source amplicons has the potential to identify systematic biases, enhance variant calling and improve the designs of future assays.

Results

We present Nimbus, a software suite for the analysis of amplicon-based sequencing data. Nimbus includes tools for data pre-processing, alignment, single nucleotide polymorphism (SNP), insertion and deletion calling, quality control and visualization. Nimbus can detect SNPs in its alignment seeds a

SUBMITTER: Brouwer RWW 

PROVIDER: S-EPMC6084620 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature