Xenon pressure dependence on the synchronized burst inhibition of rat cortical neuronal network cultured on multi-electrode arrays.

ABSTRACT: Mature rat cortical neuronal networks cultured on multi-electrode arrays (MEAs) are known to show spontaneous synchronized bursts accompanied by independent single spikes. The spontaneous synchronized bursts can be inhibited by Xe gas. In this study, we adjust the Xe gas pressure to control the amount of Xe in a neuron-cultured MEA medium. We show that the synchronized bursts cease completely within several minutes by applying Xe gas at partial pressures above 0.3 MPa. After depressurizing and purging with fresh air, the synchronized bursts recover to their original frequency. Thus, we confirmed that Xe acts as a network-activity inhibitor of the cultured neuronal network on MEAs. But below 0.3 MPa, the synchronized bursts are inhibited only partially, depending on the Xe partial pressure.

SUBMITTER: Uchida T 

PROVIDER: S-EPMC6084913 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature