Immunosuppressive protocols with tacrolimus after cryopreserved aortal allotransplantation in rats.

ABSTRACT:

Objectives and design

The aim of our study was to simulate in rats all aspects and techniques used in our new clinical program of cryopreserved alloarterial transplantation and investigate the influence of two immunosuppressive protocols with tacrolimus on acute rejection of these allografts.

Materials and methods

Cryopreserved abdominal aortic grafts were transplanted between Brown-Norway and Lewis rats. Tacrolimus (0.2 mg/kg daily) was administered from day 1 to day 30 (TAC1) or from day 7 to day 30 (TAC7), respectively. No immunosuppressed isogeneic (ISO) and allogeneic (ALO) rats combination served as control. Aortal wall infiltration by immunocompetent cells (MHC II+ cells of recipient origin) was studied on day 30 after transplantation. Flow cytometry was used for the

SUBMITTER: Spunda R 

PROVIDER: S-EPMC6084974 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature