Postsplicing-Derived Full-Length Intron Circles in the Protozoan Parasite Entamoeba histolytica.

ABSTRACT: Noncoding circular RNAs are widespread in the tree of life. Particularly, intron-containing circular RNAs which apparently upregulate their parental gene expression. Entamoeba histolytica, the causative agent of dysentery and liver abscesses in humans, codes for several noncoding RNAs, including circular ribosomal RNAs, but no intron containing circular RNAs have been described to date. Divergent RT-PCR and diverse molecular approaches, allowed us to detect bona fide full-length intronic circular RNA (flicRNA) molecules. Self-splicing reactions, RNA polymerase II inhibition with Actinomycin D, and second step of splicing-inhibition with boric acid showed that the production of flicRX13 (one of the flicRNAs found in this work, and our test model) depends on mRNA synthesis and pre-mRN

SUBMITTER: Mendoza-Figueroa MS 

PROVIDER: S-EPMC6085484 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature