Complete assembly of a dengue virus type 3 genome from a recent genotype III clade by metagenomic sequencing of serum.

ABSTRACT: Background: Mosquito-borne flaviviruses, such as dengue and Japanese encephalitis virus (JEV), cause life-threatening diseases, particularly in the tropics. Methods: Here we performed unbiased metagenomic sequencing of RNA extracted from the serum of four patients and the plasma of one patient, all hospitalized at a tertiary care centre in South India with severe or prolonged febrile illness, together with the serum from one healthy control, in 2014. Results: We identified and assembled a complete dengue virus type 3 sequence from a case of severe dengue fever. We also identified a small number of JEV sequences in the serum of two adults with febrile illness, including one with severe dengue. Phylogenetic analysis revealed that the dengue sequence belonged to genotype

SUBMITTER: Dias M 

PROVIDER: S-EPMC6085601 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature