New insights into the genic and metabolic characteristics of induced pluripotent stem cells from polycystic ovary syndrome women.

ABSTRACT:

Background

Polycystic ovary syndrome (PCOS) is a common endocrine and metabolic disorder that affects female fertility. However, with the lack of a corresponding research model, the pathology mechanism of PCOS is poorly understood. Induced pluripotent stem cell (iPSC) technology has been recognized as means to generate patient-specific stem cells for disease modeling.

Methods

The mRNA abundance of iPSCs was analyzed by RNA microarray and real-time polymerase chain reaction (RT-PCR). Karyotyping of iPSCs was performed with cytogenetic analysis. The mitochondrial respiration ability and glycolytic function were measured by the Seahorse Bioscience XF extracellular flux analyzer. The expression of iPSC-associated markers was identified by immunofluorescence and RT-PCR. The terat

SUBMITTER: Min Z 

PROVIDER: S-EPMC6085636 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature