Protease resistance of food proteins: a mixed picture for predicting allergenicity but a useful tool for assessing exposure.

ABSTRACT:

Background

Susceptibility to pepsin digestion of candidate transgene products is regarded an important parameter in the weight-of-evidence approach for allergenicity risk assessment of genetically modified crops. It has been argued that protocols used for this assessment should better reflect physiological conditions encountered in representative food consumption scenarios.

Aim

To evaluate whether inclusion of more physiological conditions, such as sub-optimal and lower pepsin concentrations, in combination with pancreatin digestion, improved the performance of digestibility protocols used in characterization of protein stability.

Methods

Four pairs of established allergens and their related non/weakly-allergenic counterparts (seed albumins, muscle tropomyosins, plant

SUBMITTER: Akkerdaas J 

PROVIDER: S-EPMC6085708 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature