Histone demethylase JMJD6 regulates cellular migration and proliferation in adipose-derived mesenchymal stem cells.

ABSTRACT:

Background

Adipose-derived mesenchymal stem cells (ADSCs) have been extensively explored as a promising therapeutic agent due to their differentiation, proliferation and migration abilities. The epigenetic mechanisms that regulate the fate of mesenchymal stem cells (MSCs) have been described in detail. However, the epigenetic modulation of ADSCs proliferation and migration is poorly understood.

Methods

The present study examined histone demethylases roles and expression by RT-PCR, as well as through siRNA screening and ChIP-qPCR assay. Cellular proliferation and migration assays were employed in shRNA-mediated JMJD6 knockdown and control ADSCs. PDE1C inhibition studies were conducted to confirm its role in JMJD6-mediated epigenetic regulation of ADSCs.

Results

The dat

SUBMITTER: Shen C 

PROVIDER: S-EPMC6085710 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature