Determining the Structural and Energetic Basis of Allostery in a De Novo Designed Metalloprotein Assembly.

ABSTRACT: Despite significant progress in protein design, the construction of protein assemblies that display complex functions (e.g., catalysis or allostery) remains a significant challenge. We recently reported the de novo construction of an allosteric supramolecular protein assembly (Zn-^C38/C81/C96R1₄) in which the dissociation and binding of Zn^II ions were coupled over a distance of 15 Å to the selective hydrolytic breakage and formation of a single disulfide bond. Zn-^C38/C81/C96R1₄ was constructed by Zn^II-templated assembly of a monomeric protein (R1, a derivative of cytochrome cb₅₆₂) into a tetramer, followed by progressive incorporation of noncovalent and disulfide bonding interactions into the protein-protein interf

SUBMITTER: Churchfield LA 

PROVIDER: S-EPMC6085756 | biostudies-literature | 2018 Aug

REPOSITORIES: biostudies-literature